Immunosurgery
Immunosurgery ko feere suɓngo ngam ittude ɓalndu yaajndu (trofoblast) e nder ɓalndu, rewrude e peeje sitotoksisite. Porotokol immunosurgery ina waɗi preincubation e antiserum, lootde ɗum e mediaaji ƴettugol ƴiiƴam ɓiɗɗo ngam ittude antibiotik, feeñnina ɗum ngam timminde, caggal ɗuum ittude trofoektoderm lysé e nder pipet. Ndee feere huutortee ko ngam seerndude ƴiiƴam nder ɓalndu blastocyst. Jokkondire ƴiiƴam trofoektoderm e epiteliyoŋ tiiɗɗo ina "reena" ICM e jokkondiral antibiotik, tawa ina waɗa ƴiiƴam ɗam waasde naatde e makromolekule.[1]
Immunosurgery ina waawi huutoreede ngam heɓde geɗe keewɗe laaɓtuɗe e nder ɓalndu e nder waktuuji seeɗa. ICM keɓaaɗo oo ina waawi ndeen huutoreede ngam wiɗtooji ƴiiƴam, ina moƴƴi huutoreede e ƴiiƴam mawɗam walla ɓiɗɗo gorko sabu ICM oo, alaa ko battini e mum so wonaa geɗe yaajɗe, ko wayi no taƴde ƴiiƴam ɗam e junngo. Kono so tawii njuɓɓudi mbaydi blastocyst ina ŋakki ko adii jarribo ngoo, ICM ina waawi heɓde feere immunologique. Nii woni, sifaa ɓiɗɗo kuutorteeɗo oo ina foti wonde darnde jarribo ngoo. Yanti heen, so huutoraade komplement ummoriiɗo e daabaaji, iwdi daabaaji ina jogii nafoore. Ɗe poti ko jogaade nokku keeriiɗo mo alaa pathogen ngam ɓeydude feere nde daaba oo heɓaani antibiotikuuji tawaaɗi e karbohidrat bakteriiji gonɗi e nder serum (ko waawi heɓde e daaba goɗɗo).[2][3][4]
Solter e Knowles ƴettii feere adannde ngam haɓaade ñawu nguu e deftere maɓɓe winndaande e hitaande 1975 "Ceerno reenngo ɓalndu e blastocyst peƴooru". Ɓe kuutorii ɗum ko adii fof ko ngam janngude ƴellitaare ɓiɗɗo. Hay so tawii ko immunosurgery woni feere ɓurnde heewde huutoreede ngam seerndude ICM, jarribooji keewɗi moƴƴinii golle ɗee, ko wayi no huutoraade laser (ko Tanaka, et al. mbaɗi) e mikroo-manipulatoruuji (ko Ding, et al. mbaɗi) Ɗeeɗoo laabi kesi keɓtinaaɗi ina usta baasal e geɗe cellal daabaaji ummoriiɗe e cellal ICM, ina mbaawi ustude baasal e geɗe cellal daabaaji e nder ɓalndu caɗeele caggal ɗuum so tawii ƴiye ɓiɗɗo oo ina njuutee e neɗɗo ngam safrude ƴiye.
Tuugnorgal
[taƴto | taƴto ɗaɗi wiki]- ↑ Häggström, Mikael (2014). "Allogeneic component to overcome rejection in interspecific pregnancy". WikiJournal of Medicine. 1 (1). doi:10.15347/wjm/2014.004.
- ↑ Cite error: Invalid
<ref>tag; no text was provided for refs named:02 - ↑ Cruz, Y. P.; Treichel, R. S.; Harsay, E.; Chi, K. D. (1993-01-01). "Mouse Blastocyst Immunosurgery with Commercial Antiserum to Mouse Erythrocytes". In Vitro Cellular & Developmental Biology. Animal. 29A (8): 671–675. doi:10.1007/BF02634557. JSTOR 4294104. PMID 8376320. S2CID 7019136.
- ↑ Nichols, Jennifer; Boroviak, Thorsten (2015). "Maximizing Clonal Embryonic Stem Cell Derivation by ERK Pathway Inhibition". Embryonic Stem Cell Protocols. Methods in Molecular Biology. 1341. pp. 1–13. doi:10.1007/7651_2015_253. ISBN 978-1-4939-2953-5. PMID 26026883.